EPR

KeyTech

EPR is the only method for the direct detection of paramagnetic species. Electron paramagnetic resonance (EPR) spectroscopy applications span across a wide range of areas from quality control to molecular research in fields such as material research, structural biology and quantum physics. EPR experiments have provided invaluable information pertaining to metalloprotein structures and to the structures and processes in photosynthesis for example.
EPR equipment located at the I2BC is a unique platform for applications in Life Sciences. Local research projects making a heavy use of EPR are mainly centered around enzymatic systems associated with oxidative stress or photosynthesis:
- Catalytic mechanism of metalloproteins with iron or manganese: photosynthetic complexes, NO-synthase, cytochrome P450, mono and bifunctional peroxidases, superoxide dismutase
- Biomimetic chemical complexes
- Metal- peptide systems involved in neurodegeneration
- Oxidative stress in Plants
- Protein radicals involved in enzyme catalysis

Equipments

The Unit currently has three continuous wave EPR spectrometers functioning at X-band (9 GHz, two spectrometers Bruker ELEXYS E500 and a spectrometer Bruker ESP300E), as well as a Bruker e-scan bench spectrometer to assay radicals at room temperature. A pulse X-band spectrometer Bruker ELEXSYS E580 with ELDOR and ENDOR capabilities is also available. Several types of cavity are available for cw X-band experiments: standard rectangular, dual-mode, high sensitivity.

Spectrometer

Frequency

Mode

Temperature

Double Resonance

e-scan

X-band –9 GHz

cw

Room T

ESP300E

X-band –9 GHz

cw

Room T

ELEXSYS E500 (2)

X-band –9 GHz

cw

≥ 4.2 K

ELEXSYS E580

X-band –9 GHz

Pulse

≥ 4.2 K

ENDOR/ELDOR

team

Alain BOUSSAC

Head of Facility

Researcher Engineer

Tania TIBILETTI

Researcher

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