Protein maturation,
cell fate and therapeutics

Unveil Molecular And Cellular Functions, Diversity And Evolution Of Essential N-Terminal Protein Modifications

Research Overview

Major co-translational N-terminal protein modification (NME, MYR, NTA)

Research highlights

It is recognized that the majority of proteins, to become functional actives, needs to undergo several modifications, which are most of the time catalyzed by specific enzymes. Recently the term of epiproteome has been coined and referred to the set of protein modifications (PTMs) made to proteins in a cellular compartment, cell or organism and in a given context. Although more than 660 different PTMs have been identified up to now, the extent of the epiproteome is still largely unknown. However, it is now clear that PTMs reflect a specific physiologic, metabolic or differentiation state of the cells or its adaptation to endogenous or exogenous stress conditions, such as the reciprocal response of the host and pathogens during infection. Thus, PTMs are necessarily of critical importance in translational research and drug development.

 

The global objective of our research is to get the picture and to provide relevant tools to better evaluate the yet underestimated but essential area of epiproteomics by focusing on N-terminal protein modifications (NPMs), as original example studied in various contexts. NPMs correspond to a variety of chemical modifications occurring on most proteins which imprinting starts in the course of their synthesis as soon as the polypeptide chains emerge while being still bound to the ribosome. NPMs involve proteolytic events including N-terminal Methionine Excision (NME) and additions such as acylations, which correspond to N-α-acetylation (NTA) or N-Myristoylation (MYR) (Fig. 1).

In the developing field of systems biology – which integrates data coming from various high-throughput technologies together with quantitative parameters describing the system (for instance extent of the modifications on the same site)  – protein speciation describing how proteins work and evolve in a given cell environment and in response to biotic and abiotic stresses is a missing link. Thus, our research aims at deeply characterizing at structural and proteomic levels the functions related to NME, MYR and NTA in a dynamic manner. In parallel, mutant lines devoid of the gene of interest and/or with inducible expression of the enzyme are expected to give answers to the physiological impact of the functions.

Research Topics

Model organisms

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team

Carmela Giglione

Group Leader

Research Director

Thierry Meinnel

Senior Researcher - CNRS

Frédéric Frottin

Researcher - CNRS

Cyril Dian

Research engineer - CNRS -

Jean-Baptiste Boyer

Project Engineer - CNRS -

Lucie Jomat

Project Engineer - CNRS -

Thomas Asensio

PhD student - UPSaclay

Jean-Baptiste Berthelot

Master Student - CNRS

Frédéric Rivière

PhD student - CNRS

Xiaofen Wu

PhD student - Gvt Chinois

team

Carmela Giglione

Group Leader

Research Director

Thierry Meinnel

Senior Researcher - CNRS

Frédéric Frottin

Researcher - CNRS

Cyril Dian

Research engineer - CNRS -

Jean-Baptiste Boyer

Project Engineer - CNRS -

Thomas Asensio

PhD student - UPSaclay

Mahmoud Abdou

Assistant engineer - CNRS

Jean-Baptiste Berthelot

Master Student - CNRS

Katia de Oliveira

BTS Student - CNRS

Rémi Dutheil

Engineer student - CNRS

Frédéric Rivière

PhD student - CNRS

Xiaofen Wu

PhD student - Gvt Chinois

Latest publications

For all the publications of the Team click on the button below.

External funding

ANR-PRCI CANMORE

(2021-2024),

ANR-PRC DynaMYT (2021-2021).

LABEX SACLAY PLANT SCIENCE.

ERACAPS

2018-2021.

FRM 2021-2022.

ARC 2021-2022.

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