I2BC publishes 200-250
articles and reviews a year
Institute for Integrative Biology of the Cell
Publications
Publications / year
0
different journals
0
fields
0
Publications / year
0
different journals
0
fields
0
Latest Publications
3888256
i2bc
1
chicago-author-date
10
date
desc
year
4613
https://www.i2bc.paris-saclay.fr/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3Afalse%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22ETW55ERX%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22M%5Cu00fcnz%20et%20al.%22%2C%22parsedDate%22%3A%222025-12-31%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%26gt%3BM%26%23xFC%3Bnz%2C%20Christian%2C%20Grant%20R.%20Campbell%2C%20Audrey%20Esclatine%2C%20et%20al.%202025.%20%26%23x201C%3BAutophagy%20Machinery%20as%20Exploited%20by%20Viruses.%26%23x201D%3B%20%26lt%3Bi%26gt%3BAutophagy%20Reports%26lt%3B%5C%2Fi%26gt%3B%204%20%281%29%3A%2027694127.2025.2464986.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1080%5C%2F27694127.2025.2464986%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1080%5C%2F27694127.2025.2464986%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Autophagy%20machinery%20as%20exploited%20by%20viruses%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christian%22%2C%22lastName%22%3A%22M%5Cu00fcnz%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Grant%20R.%22%2C%22lastName%22%3A%22Campbell%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Audrey%22%2C%22lastName%22%3A%22Esclatine%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mathias%22%2C%22lastName%22%3A%22Faure%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Patrick%22%2C%22lastName%22%3A%22Labonte%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marion%22%2C%22lastName%22%3A%22Lussignol%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Anthony%22%2C%22lastName%22%3A%22Orvedahl%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nihal%22%2C%22lastName%22%3A%22Altan-Bonnet%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ralf%22%2C%22lastName%22%3A%22Bartenschlager%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Rupert%22%2C%22lastName%22%3A%22Beale%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mara%22%2C%22lastName%22%3A%22Cirone%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lucile%22%2C%22lastName%22%3A%22Espert%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jae%22%2C%22lastName%22%3A%22Jung%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22David%22%2C%22lastName%22%3A%22Leib%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Fulvio%22%2C%22lastName%22%3A%22Reggiori%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sumana%22%2C%22lastName%22%3A%22Sanyal%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Stephen%20A.%22%2C%22lastName%22%3A%22Spector%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Volker%22%2C%22lastName%22%3A%22Thiel%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christophe%22%2C%22lastName%22%3A%22Viret%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yu%22%2C%22lastName%22%3A%22Wei%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Thomas%22%2C%22lastName%22%3A%22Wileman%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Harald%22%2C%22lastName%22%3A%22Wodrich%22%7D%5D%2C%22abstractNote%22%3A%22Viruses%20adapt%20and%20modulate%20cellular%20pathways%20to%20allow%20their%20replication%20in%20host%20cells.%20The%20catabolic%20pathway%20of%20macroautophagy%2C%20for%20simplicity%20referred%20to%20as%20autophagy%2C%20is%20no%20exception.%20In%20this%20review%2C%20we%20discuss%20anti-viral%20functions%20of%20both%20autophagy%20and%20select%20components%20of%20the%20autophagy%20machinery%2C%20and%20how%20viruses%20have%20evaded%20them.%20Some%20viruses%20use%20the%20membrane%20remodeling%20ability%20of%20the%20autophagy%20machinery%20to%20build%20their%20replication%20compartments%20in%20the%20cytosol%20or%20efficiently%20egress%20from%20cells%20in%20a%20non-lytic%20fashion.%20Some%20of%20the%20autophagy%20machinery%20components%20and%20their%20remodeled%20membranes%20can%20even%20be%20found%20in%20viral%20particles%20as%20envelopes%20or%20single%20membranes%20around%20virus%20packages%20that%20protect%20them%20during%20spreading%20and%20transmission.%20Therefore%2C%20studies%20on%20autophagy%20regulation%20by%20viral%20infections%20can%20reveal%20functions%20of%20the%20autophagy%20machinery%20beyond%20lysosomal%20degradation%20of%20cytosolic%20constituents.%20Furthermore%2C%20they%20can%20also%20pinpoint%20molecular%20interactions%20with%20which%20the%20autophagy%20machinery%20can%20most%20efficiently%20be%20manipulated%2C%20and%20this%20may%20be%20relevant%20to%20develop%20effective%20disease%20treatments%20based%20on%20autophagy%20modulation.%22%2C%22date%22%3A%222025-12-31%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1080%5C%2F27694127.2025.2464986%22%2C%22ISSN%22%3A%222769-4127%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222025-04-10T05%3A37%3A15Z%22%7D%7D%2C%7B%22key%22%3A%22BNHGSMN4%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Duqu%5Cu00e9nois%20et%20al.%22%2C%22parsedDate%22%3A%222025-12-18%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%26gt%3BDuqu%26%23xE9%3Bnois%2C%20Isoline%2C%20St%26%23xE9%3Bphanie%20Th%26%23xE9%3Bbault%2C%20Sarah%20Johari%2C%20et%20al.%202025.%20%26%23x201C%3BOptimization%20of%20the%20VSV-G%20Backbone%20for%20Amino%20Terminal%20Fusion%20with%20Nanobodies%20Allowing%20Its%20Specific%20Retargeting%20to%20HER2%20Receptors.%26%23x201D%3B%20%26lt%3Bi%26gt%3BMolecular%20Therapy.%20Oncology%26lt%3B%5C%2Fi%26gt%3B%2033%20%284%29%3A%20201065.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.omton.2025.201065%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.omton.2025.201065%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Optimization%20of%20the%20VSV-G%20backbone%20for%20amino%20terminal%20fusion%20with%20nanobodies%20allowing%20its%20specific%20retargeting%20to%20HER2%20receptors%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Isoline%22%2C%22lastName%22%3A%22Duqu%5Cu00e9nois%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22St%5Cu00e9phanie%22%2C%22lastName%22%3A%22Th%5Cu00e9bault%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sarah%22%2C%22lastName%22%3A%22Johari%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22H%5Cu00e9l%5Cu00e8ne%22%2C%22lastName%22%3A%22Raux%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Malika%22%2C%22lastName%22%3A%22Ouldali%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sandrine%22%2C%22lastName%22%3A%22Moutel%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22C%5Cu00e9cile%22%2C%22lastName%22%3A%22Lagaudri%5Cu00e8re-Gesbert%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Franck%22%2C%22lastName%22%3A%22Perez%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Aur%5Cu00e9lie%20A.%22%2C%22lastName%22%3A%22Albertini%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yves%22%2C%22lastName%22%3A%22Gaudin%22%7D%5D%2C%22abstractNote%22%3A%22Vesicular%20stomatitis%20virus%20%28VSV%29%20is%20a%20promising%20oncolytic%20virus.%20Additionally%2C%20its%20glycoprotein%20G%20%28VSV-G%29%20is%20the%20most%20commonly%20used%20envelope%20glycoprotein%20to%20pseudotype%20lentiviral%20vectors%20for%20gene%20therapy.%20However%2C%20VSV%20receptors%20%28low-density%20lipoprotein%20receptor%20%5BLDL-R%5D%20family%20members%29%20are%20ubiquitous%20and%20expressed%20at%20the%20surface%20of%20non-target%20cells%2C%20precluding%20in%20vivo%20gene%20therapy.%20Recently%2C%20we%20identified%20VSV-G%20mutants%20that%20no%20longer%20bind%20to%20LDL-R%20but%20retain%20their%20fusion%20activity.%20This%20created%20the%20opportunity%20to%20specifically%20retarget%20the%20glycoprotein%20to%20the%20desired%20receptors.%20Here%2C%20we%20engineered%20chimeric%20glycoproteins%20by%20fusing%20nanobodies%20to%20the%20amino%20terminus%20of%20VSV-G.%20Using%20experimental%20evolution%2C%20we%20identified%20two%20mutations%20in%20VSV-G%20improving%20the%20folding%20and%20functionality%20of%20chimeric%20glycoproteins%2C%20regardless%20of%20the%20nanobody%20inserted%20at%20the%20amino%20terminus.%20We%20then%20generated%20chimeric%20glycoproteins%20using%20several%20nanobodies%20targeting%20HER2%20receptor%2C%20and%20into%20these%20chimeras%2C%20we%20introduced%20additional%20mutations%20that%20abolish%20recognition%20of%20LDL-R.%20VSV%20and%20lentiviruses%20pseudotyped%20with%20these%20glycoproteins%20specifically%20infect%20cells%20expressing%20HER2.%20We%20have%2C%20therefore%2C%20identified%20VSV-G%20mutations%20that%20optimize%20its%20backbone%20to%20tolerate%20amino-terminal%20insertion%20of%20a%20nanobody%20and%20establish%20proof%20of%20concept%20that%20this%20approach%20can%20be%20used%20to%20confer%20a%20new%20tropism%20on%20VSV-G.%20This%20paves%20the%20way%20for%20the%20development%20of%20targeted%20in%20vivo%20therapies.%22%2C%22date%22%3A%222025-12-18%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.omton.2025.201065%22%2C%22ISSN%22%3A%222950-3299%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222025-10-28T08%3A27%3A40Z%22%7D%7D%2C%7B%22key%22%3A%227NE9FD5R%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Torossian%20et%20al.%22%2C%22parsedDate%22%3A%222025-12-01%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%26gt%3BTorossian%2C%20Nouritza%2C%20Marc%20Gabriel%2C%20Panagiotis%20Papoutsoglou%2C%20et%20al.%202025.%20%26%23x201C%3BReference-Free%20RNA%20Profiling%20Predicts%20Triple%20Negative%20Breast%20Cancer%20Chemoresistance%20to%20Neoadjuvant%20Treatment.%26%23x201D%3B%20%26lt%3Bi%26gt%3BNAR%20Cancer%26lt%3B%5C%2Fi%26gt%3B%207%20%284%29%3A%20zcaf036.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-ItemURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fnarcan%5C%2Fzcaf036%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fnarcan%5C%2Fzcaf036%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Reference-free%20RNA%20profiling%20predicts%20triple%20negative%20breast%20cancer%20chemoresistance%20to%20neoadjuvant%20treatment%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nouritza%22%2C%22lastName%22%3A%22Torossian%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marc%22%2C%22lastName%22%3A%22Gabriel%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Panagiotis%22%2C%22lastName%22%3A%22Papoutsoglou%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Dominika%22%2C%22lastName%22%3A%22Foretek%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Camille%22%2C%22lastName%22%3A%22Brochard%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Maud%22%2C%22lastName%22%3A%22Kamal%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Linda%22%2C%22lastName%22%3A%22Ramdani%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Constance%22%2C%22lastName%22%3A%22Lamy%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Charlotte%22%2C%22lastName%22%3A%22Lecerf%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Maral%22%2C%22lastName%22%3A%22Halladjian%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Celia%22%2C%22lastName%22%3A%22Dupain%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Josiane%22%2C%22lastName%22%3A%22Lafleur%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Adriana%22%2C%22lastName%22%3A%22Aguilar-Mahecha%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mark%22%2C%22lastName%22%3A%22Basik%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Anne%22%2C%22lastName%22%3A%22Vincent-Salomon%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christophe%5Cu00a0L%20E%22%2C%22lastName%22%3A%22Tourneau%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sergio%22%2C%22lastName%22%3A%22Roman-Roman%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Daniel%22%2C%22lastName%22%3A%22Gautheret%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Antonin%22%2C%22lastName%22%3A%22Morillon%22%7D%5D%2C%22abstractNote%22%3A%22Triple%20negative%20breast%20cancer%20%28TNBC%29%20is%20the%20most%20aggressive%20breast%20cancer%20%28BC%29%20and%20often%20affects%20young%20women.%20TNBCs%20are%20highly%20heterogeneous%20and%20do%20not%20benefit%20from%20personalized%20medicine%20at%20localized%20stages.%20Most%20TNBC%20patients%20undergo%20neoadjuvant%20chemotherapy%20%28NAC%29%20before%20surgery.%20In%20case%20of%20chemoresistance%20with%20residual%20tumor%20after%20NAC%2C%20survival%20is%20poor%20despite%20execution%20of%20complete%20tumor%20resection.%20There%20is%20currently%20no%20clinically%20useful%20biomarker%20to%20predict%20TNBC%20chemoresistance%20to%20NAC%20that%5Cu00a0would%20enable%20targeted%20therapeutic%20intensification.%20We%20analyzed%20here%20a%20unique%20cohort%20of%20106%20TNBC%20tumors%20before%20NAC%2C%20including%2058%20chemoresistant%20and%2048%20chemosensitive%20cases%2C%20from%202%5Cu00a0independent%20hospitals.%20Using%20machine%20learning%20under%20a%20nested%20cross-validation%20design%2C%20we%20obtained%20two%20transcriptomic%20signatures%20respectively%20generated%20from%20standard%20differential%20gene%20expression%20analysis%20and%20reference-free%20analysis%20of%20differential%20fragments%20of%20transcripts%2C%20without%20any%20annotation%20bias.%20This%20approach%20resulted%20in%20accurate%20signatures%20of%20TNBC%20chemoresistance%20to%20NAC.%20Gene%20ontology%20analyses%20of%20reference-free%20signatures%20highlighted%20DNA%20repair%2C%20replication%2C%20and%20metabolism%2C%20in%20agreement%20with%20current%20knowledge%20of%20TNBC%20resistance%20biology.%20In%20summary%2C%20these%20results%20show%20the%20potential%20of%20a%20reference-free%20generated%20transcriptomic%20signature%20as%20predictive%20biomarker%20of%20early%20TNBC%20chemoresistance.%22%2C%22date%22%3A%222025-12-01%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1093%5C%2Fnarcan%5C%2Fzcaf036%22%2C%22ISSN%22%3A%222632-8674%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fnarcan%5C%2Fzcaf036%22%2C%22collections%22%3A%5B%22GXCB8CXY%22%5D%2C%22dateModified%22%3A%222025-10-13T09%3A32%3A58Z%22%7D%7D%2C%7B%22key%22%3A%22IMX5EVAC%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Miranda%20et%20al.%22%2C%22parsedDate%22%3A%222025-12%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%26gt%3BMiranda%2C%20Maria%2C%20Andrea%20Mesaros%2C%20Nathalie%20Scrima%2C%20et%20al.%202025.%20%26%23x201C%3BPOLRMT%20Overexpression%20Increases%20mtDNA%20Transcription%20without%20Affecting%20Steady-State%20mRNA%20Levels.%26%23x201D%3B%20%26lt%3Bi%26gt%3BLife%20Science%20Alliance%26lt%3B%5C%2Fi%26gt%3B%208%20%2812%29%3A%20e202302563.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.26508%5C%2Flsa.202302563%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.26508%5C%2Flsa.202302563%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22POLRMT%20overexpression%20increases%20mtDNA%20transcription%20without%20affecting%20steady-state%20mRNA%20levels%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Maria%22%2C%22lastName%22%3A%22Miranda%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Andrea%22%2C%22lastName%22%3A%22Mesaros%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nathalie%22%2C%22lastName%22%3A%22Scrima%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Louise%22%2C%22lastName%22%3A%22P%5Cu00e9rard%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Irina%22%2C%22lastName%22%3A%22Kuznetsova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Martin%22%2C%22lastName%22%3A%22Purrio%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ilian%22%2C%22lastName%22%3A%22Atanassov%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Aleksandra%22%2C%22lastName%22%3A%22Filipovska%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Arnaud%22%2C%22lastName%22%3A%22Mourier%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nils-G%5Cu00f6ran%22%2C%22lastName%22%3A%22Larsson%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Inge%22%2C%22lastName%22%3A%22K%5Cu00fchl%22%7D%5D%2C%22abstractNote%22%3A%22POLRMT%20is%20the%20sole%20RNA%20polymerase%20in%20human%20mitochondria%2C%20where%20it%20generates%20primers%20for%20mitochondrial%20DNA%20%28mtDNA%29%20replication%20and%20transcribes%20the%20mtDNA%20to%20express%20genes%20encoding%20essential%20components%20of%20the%20oxidative%20phosphorylation%20%28OXPHOS%29%20system.%20Elevated%20POLRMT%20levels%20are%20found%20in%20several%20cancers%20and%20in%20mouse%20models%20with%20severe%20mitochondrial%20dysfunction.%20Here%2C%20we%20generated%20and%20characterized%20mice%20overexpressing%20Polrmt%20to%20investigate%20the%20physiological%20and%20molecular%20consequences%20of%20elevated%20POLRMT%20levels.%20Increasing%20POLRMT%20levels%20did%20not%20result%20in%20any%20pathological%20phenotype%20but%20led%20to%20increased%20exercise%20performance%20in%20male%20mice%20under%20stress%20conditions.%20Polrmt%20overexpression%20increased%20mtDNA%20transcription%20initiation%2C%20resulting%20in%20higher%20steady-state%20levels%20of%20the%20promoter-proximal%20L-strand%20transcript%207S%20RNA.%20Surprisingly%2C%20the%20abundance%20of%20mature%20mitochondrial%20RNAs%20was%20not%20affected%20by%20the%20elevated%20POLRMT%20levels.%20Furthermore%2C%20ubiquitous%20simultaneous%20overexpression%20of%20Polrmt%20and%20Lrpprc%2C%20which%20stabilizes%20mitochondrial%20messenger%20RNAs%2C%20did%20not%20increase%20steady-state%20levels%20of%20mitochondrial%20transcripts%20in%20the%20mouse.%20Our%20data%20show%20that%20POLRMT%20levels%20regulate%20transcription%20initiation%2C%20but%20additional%20regulatory%20steps%20downstream%20of%20transcription%20initiation%20and%20transcript%20stability%20limit%20OXPHOS%20biogenesis.%22%2C%22date%22%3A%222025-12%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.26508%5C%2Flsa.202302563%22%2C%22ISSN%22%3A%222575-1077%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222025-10-27T10%3A26%3A51Z%22%7D%7D%2C%7B%22key%22%3A%22TREG7D2H%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Meza-Torres%20et%20al.%22%2C%22parsedDate%22%3A%222025-12%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%26gt%3BMeza-Torres%2C%20Jazmin%2C%20Jean-Yves%20Tinevez%2C%20Aline%20Crouzols%2C%20et%20al.%202025.%20%26%23x201C%3BClostridioides%20Difficile%20Binary%20Toxin%20CDT%20Induces%20Biofilm-like%20Persisting%20Microcolonies.%26%23x201D%3B%20%26lt%3Bi%26gt%3BGut%20Microbes%26lt%3B%5C%2Fi%26gt%3B%2017%20%281%29%3A%202444411.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1080%5C%2F19490976.2024.2444411%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1080%5C%2F19490976.2024.2444411%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Clostridioides%20difficile%20binary%20toxin%20CDT%20induces%20biofilm-like%20persisting%20microcolonies%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jazmin%22%2C%22lastName%22%3A%22Meza-Torres%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jean-Yves%22%2C%22lastName%22%3A%22Tinevez%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Aline%22%2C%22lastName%22%3A%22Crouzols%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22H%5Cu00e9lo%5Cu00efse%22%2C%22lastName%22%3A%22Mary%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Minhee%22%2C%22lastName%22%3A%22Kim%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lise%22%2C%22lastName%22%3A%22Hunault%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Susan%22%2C%22lastName%22%3A%22Chamorro-Rodriguez%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Emilie%22%2C%22lastName%22%3A%22Lejal%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Pamela%22%2C%22lastName%22%3A%22Altamirano-Silva%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22D%5Cu00e9borah%22%2C%22lastName%22%3A%22Groussard%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Samy%22%2C%22lastName%22%3A%22Gobaa%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Johann%22%2C%22lastName%22%3A%22Peltier%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Benoit%22%2C%22lastName%22%3A%22Chassaing%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Bruno%22%2C%22lastName%22%3A%22Dupuy%22%7D%5D%2C%22abstractNote%22%3A%22Clinical%20symptoms%20of%20Clostridioides%20difficile%20infection%20%28CDI%29%20range%20from%20diarrhea%20to%20pseudomembranous%20colitis.%20A%20major%20challenge%20in%20managing%20CDI%20is%20the%20high%20rate%20of%20relapse.%20Several%20studies%20correlate%20the%20production%20of%20CDT%20binary%20toxin%20by%20clinical%20strains%20of%20C.%20difficile%20with%20higher%20relapse%20rates.%20Although%20the%20mechanism%20of%20action%20of%20CDT%20on%20host%20cells%20is%20known%2C%20its%20exact%20contribution%20to%20CDI%20is%20still%20unclear.%20To%20understand%20the%20physiological%20role%20of%20CDT%20during%20CDI%2C%20we%20established%20two%20hypoxic%20relevant%20intestinal%20models%2C%20Transwell%20and%20Microfluidic%20Intestine-on-Chip%20systems.%20Both%20were%20challenged%20with%20the%20epidemic%20strain%20UK1%20CDT%2B%20and%20its%20isogenic%20CDT-%20mutant.%20We%20report%20that%20CDT%20induces%20mucin-associated%20microcolonies%20that%20increase%20C.%20difficile%20colonization%20and%20display%20biofilm-like%20properties%20by%20enhancing%20C.%20difficile%20resistance%20to%20vancomycin.%20Importantly%2C%20biofilm-like%20microcolonies%20were%20also%20observed%20in%20the%20cecum%20and%20colon%20of%20infected%20mice.%20Hence%2C%20our%20study%20shows%20that%20CDT%20induces%20biofilm-like%20microcolonies%2C%20increasing%20C.%20difficile%20persistence%20and%20risk%20of%20relapse.%22%2C%22date%22%3A%222025-12%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1080%5C%2F19490976.2024.2444411%22%2C%22ISSN%22%3A%221949-0984%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222025-01-07T09%3A54%3A07Z%22%7D%7D%2C%7B%22key%22%3A%22II4K7N2A%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Royer%20et%20al.%22%2C%22parsedDate%22%3A%222025-11-26%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%26gt%3BRoyer%2C%20Alexia%20L.%20M.%2C%20%26%23xC9%3Bmeline%20Dion%2C%20Andrew%20A.%20Umansky%2C%20Mariano%20Avino%2C%20Olga%20Soutourina%2C%20and%20Louis-Charles%20Fortier.%202025.%20%26%23x201C%3BStructural%20Determinants%20of%20SlpA-Mediated%20Phage%20Recognition%20in%20Clostridioides%20Difficile.%26%23x201D%3B%20%26lt%3Bi%26gt%3BPLoS%20Pathogens%26lt%3B%5C%2Fi%26gt%3B%2021%20%2811%29%3A%20e1013724.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1371%5C%2Fjournal.ppat.1013724%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1371%5C%2Fjournal.ppat.1013724%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Structural%20determinants%20of%20SlpA-mediated%20phage%20recognition%20in%20Clostridioides%20difficile%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alexia%20L.%20M.%22%2C%22lastName%22%3A%22Royer%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22%5Cu00c9meline%22%2C%22lastName%22%3A%22Dion%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Andrew%20A.%22%2C%22lastName%22%3A%22Umansky%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mariano%22%2C%22lastName%22%3A%22Avino%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Olga%22%2C%22lastName%22%3A%22Soutourina%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Louis-Charles%22%2C%22lastName%22%3A%22Fortier%22%7D%5D%2C%22abstractNote%22%3A%22Phage%20therapy%20is%20a%20promising%20alternative%20to%20antibiotics%20for%20treating%20Clostridioides%20difficile%20infections.%20Despite%20its%20fundamental%20importance%2C%20the%20initial%20and%20most%20critical%20step%20of%20C.%20difficile%20phage%20infection%20cycle%20-%20the%20precise%20recognition%20of%20a%20host%20receptor%20-%20remains%20poorly%20understood.%20The%20surface-layer%20protein%20SlpA%20was%20previously%20identified%20as%20a%20general%20phage%20receptor%20in%20C.%20difficile%2C%20but%20the%20molecular%20determinants%20of%20phage-S-layer%20interactions%20remain%20unclear.%20We%20investigated%20the%20structural%20features%20of%20SlpA%20required%20for%20phage%20recognition%20by%20engineering%20and%20expressing%20modified%20isoforms%20in%20FM2.5%20and%20R20291%20%5Cu0394slpA%20strains.%20By%20deleting%20specific%20SlpA%20domains%20and%20creating%20chimeric%20constructs%20from%20different%20isoforms%2C%20we%20assessed%20the%20capacity%20of%20seven%20phages%20to%20adsorb%20and%20infect%20the%20complemented%20strains.%20Our%20results%20demonstrate%20that%20both%20the%20LMW%20and%20HMW%20fragments%20of%20the%20S-layer%20protein%20contribute%20to%20phage%20specificity%20in%20an%20isoform-dependent%20manner.%20In%20addition%2C%20the%20LMW%20D2%20domain%20is%20frequently%20required%2C%20but%20not%20always%20essential%20for%20productive%20infection.%20Several%20phages%20were%20also%20able%20to%20infect%20some%20of%20the%20complemented%20strains%20despite%20poor%20or%20delayed%20adsorption%2C%20highlighting%20differences%20in%20receptor%20binding.%20Interestingly%2C%20some%20phages%20adsorbed%20efficiently%20but%20failed%20to%20infect%2C%20showing%20that%20the%20capacity%20to%20bind%20to%20a%20host%20is%20not%20always%20predictive%20of%20a%20successful%20infection.%20Our%20findings%20reveal%20the%20complexity%20of%20phage-host%20interactions%20in%20C.%20difficile%20and%20provide%20new%20insights%20into%20the%20structural%20features%20of%20the%20S-layer%20protein%20that%20govern%20phage%20binding.%20These%20findings%20will%20be%20instrumental%20in%20guiding%20the%20future%20design%20of%20phage%20cocktails%20to%20target%20a%20broad%20spectrum%20of%20C.%20difficile%20clinical%20isolates.%22%2C%22date%22%3A%222025-11-26%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1371%5C%2Fjournal.ppat.1013724%22%2C%22ISSN%22%3A%221553-7374%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222025-11-27T07%3A25%3A41Z%22%7D%7D%2C%7B%22key%22%3A%22DI9ZMQNA%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Dewailly%20et%20al.%22%2C%22parsedDate%22%3A%222025-11-25%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%26gt%3BDewailly%2C%20Marie%2C%20Yoann%20Fauconnet%2C%20C%26%23xE9%3Bcile%20Ducrot%2C%20et%20al.%202025.%20%26%23x201C%3BA%20Tripartite%20Protein%20Complex%20Promotes%20DNA%20Transport%20during%20Natural%20Transformation%20in%20Firmicutes.%26%23x201D%3B%20%26lt%3Bi%26gt%3BProceedings%20of%20the%20National%20Academy%20of%20Sciences%20of%20the%20United%20States%20of%20America%26lt%3B%5C%2Fi%26gt%3B%20122%20%2847%29%3A%20e2511180122.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1073%5C%2Fpnas.2511180122%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1073%5C%2Fpnas.2511180122%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22A%20tripartite%20protein%20complex%20promotes%20DNA%20transport%20during%20natural%20transformation%20in%20Firmicutes%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marie%22%2C%22lastName%22%3A%22Dewailly%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yoann%22%2C%22lastName%22%3A%22Fauconnet%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22C%5Cu00e9cile%22%2C%22lastName%22%3A%22Ducrot%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Anne-Lise%22%2C%22lastName%22%3A%22Soulet%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nathalie%22%2C%22lastName%22%3A%22Campo%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Rapha%5Cu00ebl%22%2C%22lastName%22%3A%22Guerois%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%20Pablo%22%2C%22lastName%22%3A%22Radicella%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Patrice%22%2C%22lastName%22%3A%22Polard%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jessica%22%2C%22lastName%22%3A%22Andreani%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Calum%20H.%20G.%22%2C%22lastName%22%3A%22Johnston%22%7D%5D%2C%22abstractNote%22%3A%22Natural%20genetic%20transformation%20is%20a%20conserved%20mechanism%20of%20bacterial%20horizontal%20gene%20transfer%2C%20which%20is%20directed%20entirely%20by%20the%20recipient%20cell%20and%20facilitates%20the%20acquisition%20of%20new%20genetic%20traits%20such%20as%20antibiotic%20resistance.%20Transformation%20proceeds%20via%20the%20capture%20of%20exogenous%20DNA%2C%20its%20internalization%20in%20single%20strand%20form%20%28ssDNA%29%20and%20its%20integration%20into%20the%20recipient%20chromosome%20by%20homologous%20recombination.%20While%20the%20proteins%20involved%20in%20these%20steps%20have%20mainly%20been%20identified%2C%20the%20specific%20mechanisms%20at%20play%20remain%20poorly%20characterized.%20This%20study%20takes%20advantage%20of%20recent%20advances%20in%20structural%20modeling%20to%20explore%20the%20uptake%20of%20ssDNA%20during%20transformation.%20Using%20the%20monoderm%20human%20pathogen%20Streptococcus%20pneumoniae%2C%20we%20model%20a%20tripartite%20protein%20complex%20composed%20of%20the%20transmembrane%20channel%20ComEC%2C%20and%20two%20cytoplasmic%20ssDNA-binding%20proteins%20ComFA%20and%20ComFC.%20Using%20targeted%20mutation%20and%20transformation%20assays%2C%20we%20propose%20that%20pneumococcal%20ComEC%20features%20a%20narrow%20channel%20for%20ssDNA%20passage%2C%20and%20we%20show%20this%20channel%20is%20conserved%20in%20the%20diderm%20Helicobacter%20pylori.%20We%20identify%20key%20residues%20involved%20in%20protein-protein%20and%20protein-ssDNA%20interactions%20in%20the%20pneumococcal%20tripartite%20complex%20model%20and%20we%20show%20them%20to%20be%20crucial%20for%20transformation%20efficiency.%20Structural%20modeling%20reveals%20that%20this%20tripartite%20protein%20complex%20and%20its%20interaction%20with%20ssDNA%20are%20conserved%20in%20Firmicutes.%20Overall%2C%20this%20study%20validates%20a%20tripartite%20complex%20required%20for%20the%20internalization%20of%20ssDNA%20during%20transformation%20in%20Firmicutes%2C%20providing%20insights%20into%20the%20molecular%20mechanisms%20involved%20in%20this%20horizontal%20gene%20transfer%20mechanism%20central%20to%20bacterial%20adaptation.%20It%20also%20demonstrates%20the%20power%20of%20recent%20structural%20modeling%20techniques%20such%20as%20AlphaFold3%20as%20hypothesis%20generators%20and%20guides%20for%20designing%20experiments.%22%2C%22date%22%3A%222025-11-25%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1073%5C%2Fpnas.2511180122%22%2C%22ISSN%22%3A%221091-6490%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222025-11-21T09%3A50%3A03Z%22%7D%7D%2C%7B%22key%22%3A%22YGPQ7K8H%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Elhan%20et%20al.%22%2C%22parsedDate%22%3A%222025-11-17%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%26gt%3BElhan%2C%20Helin%2C%20Alicia%20Damm%2C%20Justin%20L.%20Korfhage%2C%20et%20al.%202025.%20%26%23x201C%3BATG2A-Mediated%20DAG%20Transfer%20Recruits%20DGAT2%20for%20Lipid%20Droplet%20Growth.%26%23x201D%3B%20%26lt%3Bi%26gt%3BNature%20Structural%20%26amp%3B%20Molecular%20Biology%26lt%3B%5C%2Fi%26gt%3B%2C%20ahead%20of%20print%2C%20November%2017.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41594-025-01689-0%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41594-025-01689-0%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22ATG2A-mediated%20DAG%20transfer%20recruits%20DGAT2%20for%20lipid%20droplet%20growth%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Helin%22%2C%22lastName%22%3A%22Elhan%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alicia%22%2C%22lastName%22%3A%22Damm%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Justin%20L.%22%2C%22lastName%22%3A%22Korfhage%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Daniel%22%2C%22lastName%22%3A%22%5Cu00c1lvarez%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mehdi%22%2C%22lastName%22%3A%22Zouiouich%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Francesca%22%2C%22lastName%22%3A%22Giordano%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Stefano%22%2C%22lastName%22%3A%22Vanni%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Thomas%20J.%22%2C%22lastName%22%3A%22Melia%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Abdou%20Rachid%22%2C%22lastName%22%3A%22Thiam%22%7D%5D%2C%22abstractNote%22%3A%22Lipid%20droplet%20%28LD%29%20growth%20mechanisms%20and%20the%20roles%20of%20LD-associated%20lipid%20transfer%20proteins%20remain%20poorly%20understood.%20Here%20we%20show%20that%20the%20autophagy%20lipid%20transfer%20protein%20ATG2A%20has%20an%20anabolic%20role%20and%20promotes%20LD%20expansion%20by%20transferring%20diacylglycerol%20%28DAG%29%2C%20triacylglycerol%20%28TAG%29%20and%20phosphatidic%20acid%2C%20from%20the%20endoplasmic%20reticulum%20to%20LDs.%20In%20ATG2A%20deficiency%2C%20synthesized%20lipids%20are%20incorporated%20inefficiently%20into%20LDs%20and%20assemble%20new%20LDs.%20In%20addition%2C%20DAG%20O-acyltransferase%202%20%28DGAT2%29%2C%20which%20synthesizes%20TAG%20and%20expands%20LD%2C%20fails%20to%20relocate%20to%20LDs.%20In%20vitro%2C%20DAG%20recruits%20DGAT2%20to%20LDs.%20These%20findings%20support%20the%20idea%20that%20ATG2A-mediated%20DAG%20transfer%20recruits%20DGAT2%20to%20LDs%2C%20promoting%20LD%20expansion.%20ATG2A%20alone%20promotes%20LD%20growth%20by%20transferring%20TAG%20and%20DAG%2C%20but%20its%20effectiveness%20in%20LD%20expansion%20is%20reduced%20when%20DGAT2%20is%20inhibited.%20This%20synergistic%20action%20with%20DGAT2%20prevents%20the%20buildup%20of%20nonmembrane%20lipids%20within%20the%20endoplasmic%20reticulum%20and%20favors%20TAG%20synthesis%20on%20the%20LD%20surface.%22%2C%22date%22%3A%222025-11-17%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41594-025-01689-0%22%2C%22ISSN%22%3A%221545-9985%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222025-11-18T10%3A02%3A05Z%22%7D%7D%2C%7B%22key%22%3A%22A8FJCCIG%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Fabbre%20et%20al.%22%2C%22parsedDate%22%3A%222025-11-14%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%26gt%3BFabbre%2C%20Alicia%2C%20Camille%20Syska%2C%20Heitor%20Gobbi%20Sebinelli%2C%20et%20al.%202025.%20%26%23x201C%3BIst2%20Promotes%20Lipid%20Transfer%20by%20Osh6%20via%20Its%20Membrane%20Tethering%20and%20Lipid%20Scramblase%20Activities.%26%23x201D%3B%20%26lt%3Bi%26gt%3BScience%20Advances%26lt%3B%5C%2Fi%26gt%3B%2011%20%2846%29%3A%20eadz2217.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1126%5C%2Fsciadv.adz2217%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1126%5C%2Fsciadv.adz2217%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Ist2%20promotes%20lipid%20transfer%20by%20Osh6%20via%20its%20membrane%20tethering%20and%20lipid%20scramblase%20activities%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alicia%22%2C%22lastName%22%3A%22Fabbre%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Camille%22%2C%22lastName%22%3A%22Syska%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Heitor%20Gobbi%22%2C%22lastName%22%3A%22Sebinelli%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Anna%22%2C%22lastName%22%3A%22Cardinal%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Maud%22%2C%22lastName%22%3A%22Magdeleine%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Noha%22%2C%22lastName%22%3A%22Al-Qatabi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Juan%20Mart%5Cu00edn%22%2C%22lastName%22%3A%22D%27Ambrosio%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22C%5Cu00e9dric%22%2C%22lastName%22%3A%22Montigny%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Guillaume%22%2C%22lastName%22%3A%22Lenoir%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alenka%22%2C%22lastName%22%3A%22%5Cu010copi%5Cu010d%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Guillaume%22%2C%22lastName%22%3A%22Drin%22%7D%5D%2C%22abstractNote%22%3A%22Lipid%20transfer%20proteins%20unevenly%20distribute%20lipids%20within%20the%20cell%2C%20which%20is%20crucial%20for%20its%20functioning.%20In%20yeast%2C%20Osh6%20transfers%20phosphatidylserine%20%28PS%29%20from%20the%20endoplasmic%20reticulum%20%28ER%29%20to%20the%20plasma%20membrane%20%28PM%29%20by%20exchange%20with%20phosphatidylinositol%204-phosphate.%20We%20investigated%20why%20its%20activity%20depends%20on%20Ist2%2C%20an%20ER-resident%20lipid%20scramblase%20that%20connects%20the%20ER%20to%20the%20PM%20via%20an%20intrinsically%20disordered%20region%20%28IDR%29.%20We%20found%20that%20Osh6%2C%20in%20a%20lipid-loaded%20state%2C%20binds%20the%20Ist2%20IDR%20with%20micromolar%20affinity%20and%20functions%20at%20ER-PM%20contact%20sites%20only%20if%20its%20binding%20site%20within%20the%20IDR%20is%20sufficiently%20distant%20from%20the%20ER%20membrane.%20We%20determined%2C%20in%20reconstituted%20contact%20sites%2C%20that%20the%20association%20of%20Osh6%20with%20the%20Ist2%20IDR%20enables%20rapid%2C%20directed%20PS%20transfer.%20We%20identified%20the%20Ist2-binding%20site%20in%20Osh6%20by%20molecular%20modeling%20and%20functional%20analyses.%20Last%2C%20we%20established%20that%20Ist2%26%23039%3Bs%20scramblase%20activity%20sustains%20Osh6-mediated%20PS%20transfer%20between%20membranes.%20Identifying%20these%20functional%20partnerships%20highlights%20why%20lipid%20transport%20processes%20are%20organized%20in%20membrane%20contact%20sites.%22%2C%22date%22%3A%222025-11-14%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1126%5C%2Fsciadv.adz2217%22%2C%22ISSN%22%3A%222375-2548%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%2283ZRAMIC%22%5D%2C%22dateModified%22%3A%222025-11-18T09%3A15%3A31Z%22%7D%7D%2C%7B%22key%22%3A%226ULH2XW9%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Harper%20et%20al.%22%2C%22parsedDate%22%3A%222025-11-13%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%26gt%3BHarper%2C%20Jon%20A.%2C%20Tim%20J.%20Cooper%2C%20Margaret%20R.%20Crawford%2C%20et%20al.%202025.%20%26%23x201C%3BMismatch%20Repair%20Disturbs%20Meiotic%20Crossover%20Control%20in%20S.%20Cerevisiae.%26%23x201D%3B%20%26lt%3Bi%26gt%3BNucleic%20Acids%20Research%26lt%3B%5C%2Fi%26gt%3B%2053%20%2821%29%3A%20gkaf1136.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fnar%5C%2Fgkaf1136%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fnar%5C%2Fgkaf1136%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Mismatch%20repair%20disturbs%20meiotic%20crossover%20control%20in%20S.%20cerevisiae%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jon%20A.%22%2C%22lastName%22%3A%22Harper%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Tim%20J.%22%2C%22lastName%22%3A%22Cooper%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Margaret%20R.%22%2C%22lastName%22%3A%22Crawford%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Laura%20J.%22%2C%22lastName%22%3A%22Hunt%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Rachal%20M.%22%2C%22lastName%22%3A%22Allison%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marie-Claude%22%2C%22lastName%22%3A%22Marsolier-Kergoat%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Bertrand%22%2C%22lastName%22%3A%22Llorente%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Matthew%20J.%22%2C%22lastName%22%3A%22Neale%22%7D%5D%2C%22abstractNote%22%3A%22Crossover%20formation%20during%20meiosis%20generates%20genetic%20diversity.%20In%20many%20species%20most%20crossovers%20display%20interference%2C%20meaning%20they%20are%20spaced%20more%20evenly%20than%20expected%20by%20chance%2C%20and%20are%20called%20class%20I%20crossovers.%20Class%20II%20crossovers%2C%20a%20minority%20pathway%2C%20are%20believed%20to%20lack%20substantial%20interference.%20Here%2C%20using%20whole-genome%20recombination%20maps%2C%20we%20examine%20the%20impact%20of%20mismatch%20repair%20%28MMR%29%20on%20the%20formation%20and%20distribution%20of%20crossovers%20in%20Saccharomyces%20cerevisiae.%20Loss%20of%20the%20MMR%20protein%20Msh2%20increases%20the%20uniformity%20of%20crossover%20distributions-an%20effect%20that%20is%20independent%20of%20changes%20in%20crossover%20frequency.%20Simulations%20indicate%20that%20this%20effect%20is%20driven%20by%20increases%20in%20the%20class%20I%20crossover%20proportion%20without%20any%20change%20in%20interference%20strength.%20Consistent%20with%20this%20view%2C%20distributions%20of%20Zip3%20foci%2C%20specific%20markers%20of%20class%20I%20crossovers%2C%20are%20unchanged%20by%20MSH2%20deletion.%20Notably%2C%20in%20wild-type%20cells%2C%20fewer%20crossovers%20arise%20in%20regions%20of%20higher%20polymorphism%20density-a%20skew%20that%20depends%20on%20both%20Msh2%20and%20Zip3.%20Taken%20together%2C%20our%20results%20indicate%20a%20dual%20influence%20of%20Msh2%20on%20recombination%3A%20suppression%20of%20class%20I%20crossovers%20in%20regions%20of%20higher%20polymorphism%20density%2C%20whilst%20unexpectedly%20promoting%20class%20II%20crossover%20formation.%20Our%20findings%20highlight%20how%20MMR%20shapes%20the%20landscape%20of%20genetic%20exchange%2C%20and%20links%20recombination%20to%20sequence%20divergence%20and%20its%20role%20in%20speciation.%22%2C%22date%22%3A%222025-11-13%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1093%5C%2Fnar%5C%2Fgkaf1136%22%2C%22ISSN%22%3A%221362-4962%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222025-11-18T09%3A16%3A54Z%22%7D%7D%5D%7D
Münz, Christian, Grant R. Campbell, Audrey Esclatine, et al. 2025. “Autophagy Machinery as Exploited by Viruses.” Autophagy Reports 4 (1): 27694127.2025.2464986. https://doi.org/10.1080/27694127.2025.2464986.
Duquénois, Isoline, Stéphanie Thébault, Sarah Johari, et al. 2025. “Optimization of the VSV-G Backbone for Amino Terminal Fusion with Nanobodies Allowing Its Specific Retargeting to HER2 Receptors.” Molecular Therapy. Oncology 33 (4): 201065. https://doi.org/10.1016/j.omton.2025.201065.
Torossian, Nouritza, Marc Gabriel, Panagiotis Papoutsoglou, et al. 2025. “Reference-Free RNA Profiling Predicts Triple Negative Breast Cancer Chemoresistance to Neoadjuvant Treatment.” NAR Cancer 7 (4): zcaf036. https://doi.org/10.1093/narcan/zcaf036.
Miranda, Maria, Andrea Mesaros, Nathalie Scrima, et al. 2025. “POLRMT Overexpression Increases mtDNA Transcription without Affecting Steady-State mRNA Levels.” Life Science Alliance 8 (12): e202302563. https://doi.org/10.26508/lsa.202302563.
Meza-Torres, Jazmin, Jean-Yves Tinevez, Aline Crouzols, et al. 2025. “Clostridioides Difficile Binary Toxin CDT Induces Biofilm-like Persisting Microcolonies.” Gut Microbes 17 (1): 2444411. https://doi.org/10.1080/19490976.2024.2444411.
Royer, Alexia L. M., Émeline Dion, Andrew A. Umansky, Mariano Avino, Olga Soutourina, and Louis-Charles Fortier. 2025. “Structural Determinants of SlpA-Mediated Phage Recognition in Clostridioides Difficile.” PLoS Pathogens 21 (11): e1013724. https://doi.org/10.1371/journal.ppat.1013724.
Dewailly, Marie, Yoann Fauconnet, Cécile Ducrot, et al. 2025. “A Tripartite Protein Complex Promotes DNA Transport during Natural Transformation in Firmicutes.” Proceedings of the National Academy of Sciences of the United States of America 122 (47): e2511180122. https://doi.org/10.1073/pnas.2511180122.
Elhan, Helin, Alicia Damm, Justin L. Korfhage, et al. 2025. “ATG2A-Mediated DAG Transfer Recruits DGAT2 for Lipid Droplet Growth.” Nature Structural & Molecular Biology, ahead of print, November 17. https://doi.org/10.1038/s41594-025-01689-0.
Fabbre, Alicia, Camille Syska, Heitor Gobbi Sebinelli, et al. 2025. “Ist2 Promotes Lipid Transfer by Osh6 via Its Membrane Tethering and Lipid Scramblase Activities.” Science Advances 11 (46): eadz2217. https://doi.org/10.1126/sciadv.adz2217.
Harper, Jon A., Tim J. Cooper, Margaret R. Crawford, et al. 2025. “Mismatch Repair Disturbs Meiotic Crossover Control in S. Cerevisiae.” Nucleic Acids Research 53 (21): gkaf1136. https://doi.org/10.1093/nar/gkaf1136.
