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Münz, Christian, Grant R. Campbell, Audrey Esclatine, et al. 2025. “Autophagy Machinery as Exploited by Viruses.” Autophagy Reports 4 (1): 27694127.2025.2464986. https://doi.org/10.1080/27694127.2025.2464986.
Meza-Torres, Jazmin, Jean-Yves Tinevez, Aline Crouzols, et al. 2025. “Clostridioides Difficile Binary Toxin CDT Induces Biofilm-like Persisting Microcolonies.” Gut Microbes 17 (1): 2444411. https://doi.org/10.1080/19490976.2024.2444411.
Paumier, Clémence, Cécile Sauvanet, and Benoît Gigant. 2025. “Microtubule Nucleation: How the NEDD1:MZT1:GCP3 Trio Captures the γ-TuRC.” The Journal of Cell Biology 224 (8): e202506019. https://doi.org/10.1083/jcb.202506019.
Da Graça, Juliane, Charlotte Thiola, Myckaëla Rouabah, et al. 2025. “ER-Endosome Contacts Generate a Local Environment Promoting Phagophore Formation.” Cell Reports 44 (7): 115993. https://doi.org/10.1016/j.celrep.2025.115993.
Overduin, Michael, Gestél C. Kuyler, Mansoore Esmaili, et al. 2025. “Design of Zwitterionic Fluorescent Polymers for Membrane Protein Solubilization into Native Nanodiscs.” Biophysical Chemistry 325 (July): 107489. https://doi.org/10.1016/j.bpc.2025.107489.
Maillot, Baptiste, Haroon Rashid, Roxanne Bercy, et al. 2025. “Nanometrology Assisted Chemical Fabrication: Direct Laser Writing of Porphyrins onto Complex Surfaces.” Nanoscale, ahead of print, July 8. https://doi.org/10.1039/d5nr00765h.
Merabishvili, Khatia, Islam Zmerli, Jana Alhoussein, Christophe Regeard, and Ali Makky. 2025. “Photoactivatable Bioinspired Nanomedicines.” Chemical Society Reviews, ahead of print, July 8. https://doi.org/10.1039/d5cs00257e.
Saye, Niang, Jihad Oumerri, Ana Andreea Arteni, Jean Luc Six, and Khalid Ferji. 2025. “Visible-Light-Driven PET-RAFT Emulsion PISA of Biobased Graft Copolymers Toward Spherical Nanoparticle Formulation.” Macromolecular Rapid Communications, July 4, e00412. https://doi.org/10.1002/marc.202500412.
Holmes, Elizabeth P., Max C. Gamill, James I. Provan, et al. 2025. “Quantifying Complexity in DNA Structures with High Resolution Atomic Force Microscopy.” Nature Communications 16 (1): 5482. https://doi.org/10.1038/s41467-025-60559-x.
Ma, Emilie, Fadma Lakhal, Eleni Litsardaki, et al. 2025. “A Large C-Terminal Rad52 Segment Acts as a Chaperone to Form and Stabilize Rad51 Filaments.” Nature Communications 16 (1): 5589. https://doi.org/10.1038/s41467-025-60664-x.
