Next Generation Sequencing

The Next Generation Sequencing (NGS) Core Facility was founded in January 2010 and is accessible to all academic and industrial users.

The Core Facility offers a broad range of services: library preparation, sequencing and primary data analysis.

It has two specific aims:
• to offer users a wide range of techniques for the preparation of DNA-seq and RNA-seq libraries and their sequencing
• to offer users bioinformatics analyses: mapping and further analyses that are previously defined with the users at the beginning of each project.

In some cases, for more complicated projects, a full collaboration can be set up between the NGS Core Facility and the users.

Process

If necessary, the project is discussed in a meeting with members of the facility
You send us by e-mail a filled request form
You receive by e-mail the corresponding quote
You send us a purchase order
Samples delivery. We carry on with quality controls.
Library preparations
Sequencing
Bioinformatics analyses (primary analysis and more)
Results are sent by email along with a Data or a Mapping Report. Data will be made available for download.
• Data storage is guaranteed for two months.
Invoice

The facility processes approximately 200 projects a year.

The NGS Core Facility is ISO 9001 certified since October 2015, and NFX 50-900 certified since November 2017.

Due to the absence of a quality manager between September 2020 and December 2020, it will not be certified from January 1st 2021, but will continue to apply Quality approach. We will apply for a re-certification in the course of 2021.

The facility is member of IBiSA and France Genomique networks.

R&D Axes

Bioinformatics and Molecular biology

Epigenetics & Epitranscriptomics : detection of modified bases by direct sequencing of DNA and RNA by ONT method.
Ultra-long reads sequencing by ONT
Single Cell sequencing and analysis by 10X Genomics method
Improved small RNA libraries for Illumina
Library preparation from ultra low input material