Crystallography

Equipment

Liquid Handling Robot: Tecan and Hamilton at 19°C

These robots are used for dispensing mother liquor solutions in the crystallization well reservoir (100-150 µl)

and setting up high volume crystallization trials (0.5 µl to 2 µl).

Tecan Genesis

Hamilton

Hamilton_bd

Crystallization Robot: Cartesian at 19°C and Mosquito (LCP) at 4°C and 19°C

These robots are used to set up low volume crystallization drops in plates prefilled by the Tecan and Hamilton robots.

Routinely, volumes of 100 nl are used for both the protein and the precipitant.

Cartesian Microsys

Mosquito (LCP)

Imaging System: RI1000 Formulatrix at 4°C and 19°C

After preparation of the crystallization trays, these are stored in a crystallization cabinet equipped with a robotized visualization system that at regular intervals takes pictures of the crystallization drops and stores them on computer for inspection.

RI1000 Formulatrix

Rates

Our prices are visible on the website GIPSI.

 

Services

  • Screening and optimization of crystallization conditions for soluble and membrane proteins
  • Freezing crystals
  • Diffraction data collection at SOLEIL synchrotron
  • Crystal structure determination and refinement
  • Structural analysis
  • AlphaFold models and analysis

Acknowledgments

Acknowledgements

Please acknowledge the crystallization platform of I2BC in your posters, presentations and articles by using the following sentence:

This work benefited from the crystallization platform of I2BC, supported by the French Infrastructure for Integrated Structural Biology (FRISBI) [ANR-10-INSB-05-05]

Citations

In case of co-authorship of platform members, please indicate this address

Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), 91198, Gif-sur-Yvette, France

Contact

Scientific executive

Solange MORERA 

O1.69.82.42.13

solange.morera@i2bc.paris-saclay.fr

Technical executive

Inès LI DE LA SIERRA-GALLAY 

O1.69.82.61.14

ines.gallay@i2bc.paris-saclay.fr

Technical executive

Technical executive

Armelle VIGOUROUX 

O1.69.82.34.72

armelle.vigouroux@i2bc.paris-saclay.fr

Technical staff

Stéphane PLANCQUEEL 

O1.69.82.61.39

stephane.plancqueel@i2bc.paris-saclay.fr

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