NMR
Nuclear Magnetic Resonance

the Swiss army knife for protein analysis:

3D structure, dynamics, protein interface, enzymatic activity and post-translational modifications at the atomic scale.

KeyTech

The I2BC NMR platform (CEA/CNRS/Univ. Paris-Saclay) focuses on the characterization of proteins, either disordered or folded, involved in cell signaling: 3D structure, dynamics, protein:protein and protein:ligand interactions, described at the atomic scale that can be reached by this spectroscopy. It is based on our expertise in the production of recombinant proteins: cell signaling proteins including DNA repair players, kinases, phosphatases, produced in bacteria, insect cells or in a cell-free environment.

We have also developed and exploited a new capability: the identification and residue-by-residue kinetic monitoring of post-translational modifications (PTMs). This real-time NMR approach provides a description of multiple neighboring or degenerate patterned modifications as a function of time in the presence of modifying enzymes as well as in complex media as cell extracts. It can be applied to virtually any type of PTMs.

R&D Axes

Combining NMR together with X-ray Crystallography and Electron Microscopy analyses: interaction of disordered and folded proteins with their partners within essential cell signaling pathways