Function and architecture
of macromolecular assemblies
The FAAM team is studying the mechanisms involved in biological processes at the molecular level using structural and biochemical techniques. We apply X-ray crystallography and cryo-EM to analyse the atomic structure of target proteins and complexes and also use SAXS to study the conformation of these complexes in solution. The team is focusing on the assembly of protein complexes involved in a variety of cellular processes. The team’s main research projects concern genome replication and the cell-cell communication in bacteria, maturation of tRNAs and avirulence proteins produced by pathogenic fungi. In addition, our expertise in cloning, protein expression, purification and determination of 3D structures is exploited to carry out a considerable number of collaborative projects.
Biochemistry and Molecular Biology
- Cloning, Site-directed mutagenesis, CRIPSR-Cas9 (yeast)
- Production of recombinant proteins (expression in bacterial and eukaryotic hosts, fermentation)
- Protein and protein complex purification
- Enzymology studies
- Interaction measurement (SEC-MALS, ITC, DSC, SPR)
- X-ray crystallography (Regular access to SOLEIL and ESRF synchrotron facilities)
- Small-angle X-ray scattering (SAXS)
- Cryo-electron microscopy
You can find the latest publications at the end of each specific topic page.
For all the publications of the Team click on the button below.
ANR-15-CE12-0003-01 (sRNA-Fit, 2017-2021)
ANR-19-CE12-0006-01 (RRARE, 2019-2023)].