Function and architecture
of macromolecular assemblies

Presentation

The FAAM team is studying the mechanisms involved in biological processes at the molecular level using structural and biochemical techniques. We apply X-ray crystallography and cryo-EM to analyse the atomic structure of target proteins and complexes and also use SAXS to study the conformation of these complexes in solution. The team is focusing on the assembly of protein complexes involved in a variety of cellular processes. The team’s main research projects concern genome replication and the cell-cell communication in bacteria, maturation of tRNAs and avirulence proteins produced by pathogenic fungi. In addition, our expertise in cloning, protein expression, purification and determination of 3D structures is exploited to carry out a considerable number of collaborative projects.

Methodologies

Biochemistry and Molecular Biology

  • Cloning, Site-directed mutagenesis, CRIPSR-Cas9 (yeast)
  • Production of recombinant proteins (expression in bacterial and eukaryotic hosts, fermentation)
  • Protein and protein complex purification
  • Enzymology studies
  • Interaction measurement (SEC-MALS, ITC, DSC, SPR)

Structural Biology

  • Crystallogenesis
  • X-ray crystallography (Regular access to SOLEIL and ESRF synchrotron facilities)
  • Small-angle X-ray scattering (SAXS)
  • Cryo-electron microscopy

Topics

team

Herman Van Tilbeurgh

Team Leader

Research Director

Sophie Cheruel

PhD

Sylvie Nessler

PhD

Herman Van Tilbeurgh

PhD

Ines Gallay

PhD

Karine Blondeau

PhD

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Technician

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Technician

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Technician

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Technician

Latest publications

You can find the latest publications at the end of each specific topic page. 

For all the publications of the Team click on the button below.

External funding

ANR-15-CE12-0003-01 (sRNA-Fit, 2017-2021)
ANR-19-CE12-0006-01 (RRARE, 2019-2023)].

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