Regulatory RNAs in Clostridia

The role of non-coding RNAs in Clostridium difficile physiology and regulatory network

During infection, bacteria reprogram their gene expression in response to environmental constraints. Non-coding RNAs play key roles in the regulation of adaptive responses. Clostridia largely use complex RNA-based mechanisms. We are interested in the roles of regulatory RNAs in the pathophysiology of major human enteropathogen Clostridium difficile.

Clostridium difficile is a Gram-positive, strictly anaerobic spore-forming bacterium. This opportunistic pathogen is the leading cause of nosocomial diarrhea in adults in industrialized countries. Risk factors are age and antibiotics that by its action on the intestinal flora facilitate the emergence of C. difficile in the gut. The proportion of severe forms of C. difficile infections is currently increasing worldwide. However, the mechanisms that control virulence remain largely unexplored. Metabolic adaptations, motility, efficiency of adhesion, the ability to form biofilms, sporulate, germinate or resist to stresses are among the important processes during the infection process. Understanding the mechanisms controlling the emergence and success of this enteropathogen is of prime importance. During infection, bacteria reprogram the expression of their genes in response to environmental stresses. Recent studies of the bacterial transcriptome showed the presence of a large number of non-coding RNAs (ncRNAs). These regulatory RNAs play a key role in the regulation of adaptive responses and in many metabolic, physiological and pathogenic processes. The Clostridia belong to an ancient group of bacteria that uses complex RNA-based regulatory mechanisms to finely control their gene expression.

This project is built upon our recent genome-wide identification of a great number (more than 200) and a large diversity of potential regulatory RNAs in C. difficile. By combining in silico analysis, RNAseq and genome-wide promoter mapping we have identified ncRNAs in intergenic regions, cis-antisense RNAs, riboswitches (5’-cis-regulatory elements) and trans riboregulators requiring the RNA chaperone protein Hfq (Soutourina et al, PLoS Genetics, 2013 ; Soutourina, Current Opinion Microbiol, 2017 ; Soutourina et al, Frontiers in Microbiology, 2020). These ncRNAs might play important roles in the control of gene expression during the C. difficile infection cycle including metabolic adaptations, biofilm formation, stress responses, defence mechanisms and sporulation. Our goal is to determine the biological roles of regulatory RNAs and to uncover the molecular mechanisms of RNA-based regulations employed by C. difficile outside and inside the host. We are particularly interested in original aspects of RNA-based control in C. difficile and will focus on i) the function and regulation of C. difficile CRISPR (clustered regularly interspaced short palindromic repeats)-Cas system for defence against foreign DNA and type I toxin-antitoxin systems; ii) the role of the c-di-GMP signalling pathway mediated by ncRNAs in cellular processes associated with community behaviour; iii) the RNA chaperone protein Hfq-dependent ncRNA network controlling key steps of the C. difficile infection cycle and the role of ncRNAs specific to the epidemic strain 027. We propose an integrative approach to address the mechanisms controlling the C. difficile pathogenesis at the molecular, cellular and community levels (Soutourina, Current Opinion Microbiol, 2017). After the identification of the molecular players in this RNA-based control: regulatory RNAs, the RNA chaperone protein Hfq and specific ribonucleases, we will look at the dynamics of expression of these regulatory elements during C. difficile infection cycle and their role for the C. difficile interactions with other components of gut communities including numerous bacteriophages.

Topics

team

Olga SOUTOURINA

Group Leader

Professor Paris-Saclay University

Johann PELTIER

Assistant Professor Paris-Saclay University

Emilie LEJAL

Postdoctoral researcher

Ana OLIVEIRA PAIVA

Postdoctoral researcher

Polina MUZYUKINA

PhD student

Emma PIATTELLI

PhD student

Victor KREIS

PhD student

Lorna CUPIT

Intern

Baptiste ORTHEAU

Intern

Latest publications

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External funding

ANR (CloSTARn, DIFFICROSS, BactoCresolDiab)

Junior IUF member support

Vernadski PhD fellowships – Joint Russian-French PhD program

DIM-1HEALTH

Collaborations

International collaborators

E. Semenova, K. Severinov (Rutgers University, USA)
L.-C. Fortier, O. Sekulovic, J. Garneau (Sherbrooke University, Canada)
L. Huang, D. Lilley (University of Dundee, UK)
L. Huang (Sun Yat-sen University, Guangzhou, China)
K. Severinov, E. Savitskaya, A. Shiryaeva (Skoltech, Russia)
A. Maikova, A. Vasileva (St Petersburg Polytech University, Russia)
F. Matsuda (Kyoto University, Japan)

National collaborators

E. Hajnsdorf (IBPC, Paris), I. Poquet (Micalis, Jouy-en-Josas)
M. Monot, I. Martin-Verstraete, B. Dupuy (Institut Pasteur, Paris)
C. Janoir, I. Kansau, C. Denève-Larrazet, J-C. Marvaud (UBaPS/Micalis, Châtenay-Malabry)
F. Barbut (Nat Ref Lab for CD, Saint Antoine Hospital, Paris)
D. Gauguier (INSERM, Paris)
D. Gautheret, C. Toffano (I2BC)

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